![]() ![]() ![]() Under suggested conditions, BLUelf Prestained Protein Ladder resolves 13 major bands in polyacrylamide gel with appropriate buffers and after Western blotting to nitrocellulose membrane. The BLUelf Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating protein size and validating Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes. The 13 recombinant proteins are covalently coupled with blue chromophore, while 1 green band at 25 kDa and a red band at 75 kDa serve as reference bands. Western blot analysis of HA-epitope tag was performed using samples from Thermo Scientific Pierce HA-Tag IP/Co-IP Kit with Thermo Scientific SuperSignal Molecular Weight Protein Ladder in Lane 1, an HA-tagged positive control lysate in Lane 2, unbound flow. Someone recently told me that I should add Laemmli buffer to my ladder so that it is loaded at an equal. I never used to have an issue with this protein but recently I am getting faint bands during. The BLUelf Prestained Protein Ladder is a combination of 13 pre-stained proteins with molecular weights from 5 to 245 kDa. Use of SuperSignal molecular weight marker for chemiluminescent western blot detection. When running a western blot, I usually load less ladder (6uL) than any of my samples (20uL). I am using -actin as a loading control for my western blots to enable quantification of proteins. Why do I see my protein ladder standard on my developed western blot Im attempting to use a western blot to detect the presence of an endogenous transcription factor. 2.5 μl per well for general Western transferring. Use 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. Custom Recombinant Antibody (rAbs) Services ScanLaterTM Western Blot Protein Ladder Reagent.Annexin V-FITC Apoptosis Detection Kits. ![]()
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